Materials
– selective plates (e.g. LB-Amp)
– plasmid DNA
– Stbl3 E.coli (Invitrogen #C7373-03)
– 42oC water bath
– LB media
– SOC media (Invitrogen #15544-034)
– 37oC incubator
– 37oC incubator/shaker
– flame, streaker, 95% EtOH
– tips, pipets, tubes, ice, etc
Procedure
