1M Tris pH 6.8 6.25ml
10% SDS 20ml

Bring volume to a total of 100ml.
Add 50µl BME before use
Soak membrane for 30min at 50oC while shaking in stripping buffer
Wash membrane 2 X 10 min in TTBS (Tris +Tween)
Continue with block and then primary antibody.
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