Oil Red O quantification

  1. Remove all water after imaging cells.
  2. Place 6 well dishes (or other culture vessel) into 37°C incubator for 10-15 minutes.
  3. Wait until well is completely dry (no water left at all!)
  4. Add 300uL 100% isopropanol/6 well plate and wash stain off the cells, collecting from the top of the well to the bottom.
  5. Transfer isopropanol  to 96 well plate
  6. Read absorbance of 96 well plate at 510nm.
  7. To calculate the stain compare the differentiated absorbance to the undifferentiated absorbance (i.e. Abs 510nm diff/Abs 510nm undiff).