Drug Stocks for Mammalian Cells
|
|
|
Stock concentration |
Storage |
|---|---|---|---|
| 1 | Neomycin (G418) | 500 mg/ml | -20oC |
| 2 | Puromycin | 2.0 mg/ml | -20oC |
| 3 | Hygromycin | 100 mg/ml | 4oC T.C. |
| 4 | Zeocin | 100 mg/ml | -20oC |
| 5 | Blasticidin | 10 mg/ml | -20oC |
Information on ordering and aliquoting :
Neomycin (G418, Geneticin) – Life Tech. 11811-031 5g $265.90. Comes lyophilized, store at RT. Dissolve in PBS-. Usually, it’s activity is ~730µg/mg in which case, resuspend in 7.3 ml PBS for a 500 mg/ml stock.
Puromycin – Sigma P7255 100 mg $113.60. Dissolve in 50 ml PBS- for a final conc. of 2 mg/ml.
Hygromycin – Sigma H3274 1 g $263.50 comes lyophilized, store at 4oC. Add 8 ml H2O and it rapidly goes into solution. Bring volume to 10 ml for a final conc. of 100 mg/ml. Make 500 µl aliquots and store at 4oC in dark.
Zeocin – Invitrogen R250-05 5g $725 Comes as a liquid at 100 mg/ml.
Blasticidin S HCL – Invitrogen R210-01 50mg $135. Dissolve in 5 ml H2O for a final conc. of 10mg/ml.
