DMEMF12 Mammary Epithelial Growth Media
DME/F12 – Mammary Epithelial Growth Media 11/00
Make a 1:1 mixture of DME and F12 media
Add these supplements to a 500 ml bottle of DME/F12; 1 tube of each.
|
|
|
Final conc. |
|---|---|---|
| 1. hEGF | 0.5 ml of 10 µg/ml | 10 ng/ml |
| 2. Hydrocortisone | 0.5 ml of 0.5 mg/ml | 0.5 µg/ml |
| 3. Insulin | 1 ml of 5 mg/ml | 10 µg/ml |
| 4. Pen/Strep/Fungizone | 5 ml |
Information on ordering and aliquoting the supplements:
hEGF – Sigma E-9644 0.2 mg $125.50 enough for 40 bottles. Dissolve in 20 ml 10mM Acetic Acid, 0.1% BSA make 500 µl aliquots of 10 µg/ml.
Hydrocortisone – Sigma H-0888 1g $16.10. Dissolve in 95% Ethanol and make 500 µl aliquots. 100mg/200ml 95% Ethanol for a final conc. of 0.5 mg/ml.
Insulin – Sigma I-2767 100 mg $89.20 enough for 20 bottles. Dissolve in 20 ml 0.005 N HCL and make 1ml aliquots 5 mg/ml. Conc. HCL is 11.6 N. Make 1 N HCL and then add 100 µl to 20 ml H2O.
