- Specimen origin – precise anatomical size
- Volume of specimen
- Physical appearance of specimen – colour, viscosity, whether it is homogenous or contains tissue fragments or blood
DETERMINE THE CELL COUNT
The concentration chosen should allow enough space for cells to form a monolayer with minimum overlap, but not leave too much space between cells.
Average cells (diameter 10-12 microns) produce excellent Cytospin preparations at cell densities of 1×106 cells per ml. Larger cells will require lower concentrations; smaller cells, cell organelles or bacteria will require higher concentrations.
CONCENTRATE OR DILUTE THE SPECIMEN AS REQUIRED
Page 94 (to concentrate);
Page 95 (to dilute)
LOAD SHANDON CYTOSPIN SAMPLE CHAMBERS
Page 40-44 and page 95
Place the EZ Cytofunnels into the sealed head. Make sure they are distributed evenly so that the Shandon Cytospin 4 is not out of balance.
Load the EZ Cytofunnels after they have been inserted into the sealed head.
Do not place more than 0.5ml of a sample in an EZ Cytofunnel sample chamber with white filter card (0.4ml for an EZ Cytofunnel sample chamber with brown filter card or 6ml maximum in an EZ Megafunnel). Make sure that the sample is deposited directly into the bottom of the sample chamber – do not allow the sample to drip down the sides of the chamber.